Animal models

Diomune SL has resources and proven experience in fully controlled and standardized in vivo studies. This includes small laboratory animals for preclinical studies and other veterinary domestic species for both preclinical and clinical trials.

  • Sepsis: we currently have 3 major animal models for the study of sepsis:

– Toxic shock model by administration of LPS.

– Model of Escherichia coli septicemia.

– Cecal Ligation and Puncture (CLP) sepsis model.

  • Leishmaniasis: in a murine model of leishmaniasis (Leishmania major), we have a genetically modified Leishmania strain expressing cherry fluorescent protein (CFP), which allows high sensitivity studies with the IVIS   technology. System analysis of high-resolution images allows to study with great detail the evolution of the   disease without sacrificing the animals.
  • Inflamation and Immunology: Diomune has an extensive catalog for the study of innate and acquired immune responses in murine and human systems, allowing for study either with primary cultures or cell lines. In vivo services are offered in collaboration with the group of Dr. Manuel Fresno at the Centro de Biología Molecular Severo Ochoa (CBMSO). These services are based on models well accepted by the international scientific community and are adapted for preclinical studies of new molecules. In addition to the in vivo toxicity tests, we offer the following animal models:

– Acute Inflammation carrageenan.

– Rheumatoid arthritis (mice strain Dba1j).

– Thioglycolate-induced peritonitis (in vivo migration of neutrophils).

– Proliferation of T and B lymphocytes (BrdU staining).

– Colon cancer associated with colitis.

– Orthotopic tumors.

– Psoriasis.

– Simulation of hepatic cirrhosis.

– Asthma.

– Other specific models (please request information).

  • Metabolic Syndrome and Obesity: the metabolic syndrome is a clinical entity  that appears with large                phenotypic variations in humans. Genetics and  the environment both play important roles in the development of the metabolic syndrome. We have a specific platform for screening and study of compounds directed to different molecular targets involved in the pathophysiology of metabolic syndrome:

– Experimental models of diabetes.

– Experimental models of diet-induced obesity.

– Genetic models of diabetes.

– Atherosclerosis by hypercholesterolemic diet.

  • Hepatic Cirrhosis: we have models for the simulation of rat liver cirrhosis by blood inlet difficulty; prehepatic, by total or partial ligation of the portal vein and triple stenosing ligation site; intrahepatic by inhaled carbon tetrachloride, or hill deficit by dimethyl nitrosamine, or by ligation of the hepatic inferior vena cava.
  • Pulmonary hypertension induced by monocrotaline injection.
  • Postnatal hypoxia.
  • Endotoxemia (LPS).
  • Acute lung injury (LPS intratracheal).
  • Spontaneously hypertensive rat.
  • Diabetic Rat (type I induced by streptozotocin injection).
  • Hemodynamics in rat. Registration of systemic and pulmonary arterial pressure (open chest), volume per minute, frequency, O2 saturation.

All models are accompanied by strict validation and control systems:

– Microbiological tests.

– Determinations of glucose and lactic acid in serum.

– Study of cytokines in the culture supernatant.

– Studies of cell proliferation.

– Studies of apoptosis.

– Studies of evolution of cell subpopulations (CD3 +, CD4 +, CD8 +, MHCII, CD11, CD19,  etc…) by Flow Cytometry of peripheral blood and spleen.

– Study of somatic organ indices.

– Full Biochemistry.