Design of diagnostic kits

In Diomune we design molecular diagnostic kits based on the specific needs of our customers. Each designed kit is accompanied by a strict validations including determination of detection limit, sensitivity and specificity.

The design of molecular diagnostic kits Diomune offers are based on:

  • Detection of nucleic acids: polymerase chain reaction (quantitative PCR and conventional PCR)
    – Genotyping.
    – Analysis of polymorphisms.
    – Genomics (microarrays).
    – Expression of miRNAs.
    – Gene silencing siRNA.
  • · Immunoassays
    – Immunohistochemistry and immunocytochemistry. Localization of proteins in cells and tissue sections.
    – Western blot.
    – Protein-protein interactions (co-immunoprecipitation).
    – Two-dimensional electrophoresis.
    – Enzyme immunoassay (ELISA).